A fast method for cloning of specific cDNA. Cloning of human prolactin cDNA
نویسندگان
چکیده
منابع مشابه
A simplified, orientation-specific cDNA cloning strategy.
D) DIGESTION WITH Hind III AND EcoRI PROTOCOL, a) cDNA synthesis by Reverse Transcriptase is initiated with oligo(dT) according to Gubler and Hofmann (1) . b) The double stranded cDNA is methylated, and (c) a synthetic CCTTGAATTCAAGCNH oligonucleotlde pGCTTGAATTCAAGC CCAACTTAACTTCCNN is added. This generates a Hindlll recognition sequence (AAGCTT) when ligated to the oligo (dA) track at the 3 t...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1989
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.0000fd